FEBS Letters
Volume 582, Issue 18 , Pages 2753-2760, 6 August 2008

The roles of plant dsRNA-binding proteins in RNAi-like pathways

Edited by Shou-Wei Ding

  • Shaun J. Curtin

      Affiliations

    • CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia
    • Charles Sturt University, Wagga Wagga, NSW 2678, Australia
    • ,
  • John M. Watson

      Affiliations

    • CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia
    • ,
  • Neil A. Smith

      Affiliations

    • CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia
    • ,
  • Andrew L. Eamens

      Affiliations

    • CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia
    • ,
  • Chris L. Blanchard

      Affiliations

    • Charles Sturt University, Wagga Wagga, NSW 2678, Australia
    • ,
  • Peter M. Waterhouse

      Affiliations

    • CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia
    • University of Sydney, NSW 2006, Australia
    • Corresponding Author InformationCorresponding author. Address: CSIRO Plant Industry Canberra, P.O. Box 1600, ACT 2601, Australia. Fax: +61 2 62465000.

Received 17 June 2008; received in revised form 4 July 2008; accepted 4 July 2008. published online 14 July 2008.

Abstract 

Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.

Keywords: Double-stranded RNA, RNA binding protein, RNA interference, Gene silencing pathway

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PII: S0014-5793(08)00584-X

doi:10.1016/j.febslet.2008.07.004

FEBS Letters
Volume 582, Issue 18 , Pages 2753-2760, 6 August 2008

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