NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

Stenbaek, Anne; Hansson, Andreas; Wulff, Ragna Peterson; Hansson, Mats; Dietz, Karl-Josef; Jensen, Poul Erik

doi:10.1016/j.febslet.2008.07.006

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Volume 582, Issue 18 , Pages 2773-2778, 6 August 2008

NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

Edited by Miguel De la Rosa

Anne Stenbaek
- VKR Research Centre “Pro-Active Plants”, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
Andreas Hansson
- Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden
- Present address: Carlsberg Research Center, Gamle Carlsberg Vej 10, 2500 Copenhagen Valby, Denmark.
Ragna Peterson Wulff
- Department of Biochemistry, Lund University, Box 124, SE-22100 Lund, Sweden
Mats Hansson
- Department of Biochemistry, Lund University, Box 124, SE-22100 Lund, Sweden
Karl-Josef Dietz
- University of Bielefeld, Department of Plant Physiology and Biochemistry W5, D-33501 Bielefeld, Germany
Poul Erik Jensen
- VKR Research Centre “Pro-Active Plants”, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
- Corresponding author. Fax: +45 3533 3333.

Received 20 June 2008; received in revised form 2 July 2008; accepted 6 July 2008. published online 14 July 2008.

Abstract

The chloroplast-localized NADPH-dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg–protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g. under chloroplast biogenesis.

Abbreviations: ALA, 5-aminolevulinic acid, Chlide, chlorophyllide, H₂O₂, hydrogen peroxide, MgP-MME, Mg–protoporphyrin monomethyl ester, MgP, Mg–protoporphyrin IX, NTRC, NADPH-dependent thioredoxin reductase, Prx, peroxiredoxin, Pchlide, protochlorophyllide, PPIX, protoporphyrin IX, ROS, reactive oxygen species