Protein tyrosine phosphatase receptor-type O (PTPRO) is co-regulated by E2F1 and miR-17-92

Xu, Xin; Hong, Yan; Kong, Chenfei; Xu, Liang; Tan, Jiang; Liang, Qian; Huang, Baiqu; Lu, Jun

doi:10.1016/j.febslet.2008.07.017

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Volume 582, Issue 19 , Pages 2850-2856, 20 August 2008

Protein tyrosine phosphatase receptor-type O (PTPRO) is co-regulated by E2F1 and miR-17-92

Edited by Angel Nebreda

Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

Received 21 April 2008; received in revised form 4 July 2008; accepted 10 July 2008. published online 21 July 2008.

Abstract

PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S phase in synchronized HeLa cells and in vitro PTPRO promoter activity is high in early S phase while the PTPRO 3′UTR reporter activity is low in late S phase. This study provides evidence that the PTPRO gene is co-regulated by both E2F1 and miR-17-92.

Keywords: PTPRO, E2F1, miR-17-92