FEBS Letters
Volume 582, Issue 19 , Pages 2875-2881, 20 August 2008

Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

Edited by Michael R. Bubb

  • Konstantin G. Chernov

      Affiliations

    • Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    • Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829, Evry 91025, France
    • Equal contribution.
    • ,
  • Patrick A. Curmi

      Affiliations

    • Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829, Evry 91025, France
    • Equal contribution.
    • ,
  • Loic Hamon

      Affiliations

    • Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829, Evry 91025, France
    • ,
  • Alain Mechulam

      Affiliations

    • Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829, Evry 91025, France
    • ,
  • Lev P. Ovchinnikov

      Affiliations

    • Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    • ,
  • David Pastré

      Affiliations

    • Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829, Evry 91025, France
    • Corresponding Author InformationCorresponding author. Fax: +33 1 69 47 02 19.

Received 17 April 2008; received in revised form 25 June 2008; accepted 11 July 2008. published online 22 July 2008.

Abstract 

A significant fraction of mRNAs is known to be associated in the form of mRNPs with microtubules for active transport. However, little is known about the interaction between mRNPs and microtubules and most of previous works were focused on molecular motor:microtubule interactions. Here, we have identified, via high resolution atomic force microscopy imaging, a significant binding of mRNA to microtubules mediated by two major mRNP proteins, YB-1 and PABP. This interaction with microtubules could be of critical importance for active mRNP traffic and for mRNP granule formation. A similar role may be fulfilled by other cationic mRNA partners.

Keywords: Microtubule, RNA, RNP, Atomic force microscopy, YB-1, PABP

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PII: S0014-5793(08)00607-8

doi:10.1016/j.febslet.2008.07.019

FEBS Letters
Volume 582, Issue 19 , Pages 2875-2881, 20 August 2008

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