Microtubule-associated Protein 1B Light Chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells

Lee, So-Youn; Kim, Jung-Woong; Jeong, Mi-Hee; An, Joo-Hee; Jang, Sang-Min; Song, Ki-Hyun; Choi, Kyung-Hee

doi:10.1016/j.febslet.2008.07.021

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Volume 582, Issue 19 , Pages 2826-2832, 20 August 2008

Microtubule-associated Protein 1B Light Chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells

Edited by Varda Rotter

Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University, 221, Heuksuk-Dong, Dongjak-Ku, Seoul 156-756, Republic of Korea

Received 16 May 2008; received in revised form 12 July 2008; accepted 14 July 2008. published online 24 July 2008.

Abstract

The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses and can trigger apoptosis in many cell types, including neurons. In this study, we have shown that the Microtubule-Associated Protein 1B (MAP1B) light chain can interact with the tumor suppressor p53. We also demonstrate that both p53 and the MAP1B light chain (MAP1B-LC1) alter their localization from the cytoplasm to the nucleus when neuroblastoma cells, SH-SY5Y, are treated with doxorubicin. Additionally, we demonstrate that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter construct driven by the p21 promoter. Consequently, MAP1B-LC1 binds to p53 and this interaction leads to the inhibition of doxorubicin-induced apoptosis in SH-SY5Y cells.