Loss of peroxisome function triggers necrosis

Jungwirth, Helmut; Ring, Julia; Mayer, Tanja; Schauer, Alexandra; Büttner, Sabrina; Eisenberg, Tobias; Carmona-Gutierrez, Didac; Kuchler, Karl; Madeo, Frank

doi:10.1016/j.febslet.2008.07.023

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Volume 582, Issue 19 , Pages 2882-2886, 20 August 2008

Loss of peroxisome function triggers necrosis

Edited by Vladimir Skulachev

Helmut Jungwirth
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Julia Ring
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Tanja Mayer
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Alexandra Schauer
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Sabrina Büttner
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Tobias Eisenberg
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Didac Carmona-Gutierrez
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
Karl Kuchler
- Medical University Vienna, Christian Doppler Laboratory for Infection Biology, Max F. Perutz Laboratories, Campus Vienna Biocenter, A-1030 Vienna, Austria
Frank Madeo
- Institute of Molecular Biosciences (IMB), University of Graz, A-8010 Graz, Austria
- Corresponding author. Fax: +43 316 3809898.

Received 18 June 2008; received in revised form 11 July 2008; accepted 14 July 2008. published online 24 July 2008.

Abstract

Disturbance of peroxisome function can lead to various degenerative diseases during ageing. Here, we show that in yeast deletion of PEX6, encoding a protein involved in a key step of peroxisomal protein import, results in an increased accumulation of reactive oxygen species and an enhanced loss of viability upon acetic acid treatment and during early stationary phase. Cell death of ageing-like yeast cells lacking PEX6 does not depend on the apoptotic key players Yca1p and Aif1p, but instead shows markers of necrosis. Thus, we conclude that loss of peroxisomal function leads to a form of necrotic cell death.

Abbreviations: H₂O₂, hydrogen peroxide, DHE, dihydroethidium, ROS, reactive oxygen species, PI, propidium iodide, wt, wild-type