FEBS Letters
Volume 582, Issue 20 , Pages 3033-3036, 3 September 2008

Denitration of L-type calcium channel

Edited by Barry Halliwell

Department of Pharmacology and Toxicology, Virginia Commonwealth University, Medical College of Virginia Campus, 1112 East Clay Street, Richmond, VA 23298, United States

Received 5 June 2008; received in revised form 23 July 2008; accepted 25 July 2008. published online 01 August 2008.

Abstract 

Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCav1.2b. We report here that Cav1.2 is also subject to “denitration”. Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced nitration of the carboxy terminus of Cav1.2 in a 1D gel assay. Tyrosine phosphorylation of the calcium channel by c-src kinase was blocked by nitration but reversed by pretreatment with RAW264.7 cell lysates. These findings indicate that denitration may be a physiological mechanism to restore cellular excitability during inflammation.

Keywords: Nitric oxide, Tyrosine, Nitration, Ion channel, Src kinase, Smooth muscle

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PII: S0014-5793(08)00643-1

doi:10.1016/j.febslet.2008.07.042

FEBS Letters
Volume 582, Issue 20 , Pages 3033-3036, 3 September 2008

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