FEBS Letters
Volume 582, Issue 20 , Pages 2998-3004, 3 September 2008

Nuclear localization of profilin III–ArpM1 complex in mouse spermiogenesis

Edited by Daniela Ruffell

  • Yuki Hara

      Affiliations

    • Present addresses: Cell Architecture Laboratory, Center for Frontier Research, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
    • ,
  • Kazuo Yamagata

      Affiliations

    • Corresponding Author InformationCorresponding author. Present address: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN-Kobe, Minatojima-Minamimachi 2-2-3, Chuo-Ku, Kobe City, Hyogo 651-0047, Japan. Fax: +81 78 306 3095.
    • ,
  • Kei Oguchi
    • ,
  • Tadashi Baba

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

Received 10 April 2008; received in revised form 4 July 2008; accepted 22 July 2008. published online 06 August 2008.

Abstract 

Actin-related proteins (Arps) have been reported to be localized in the cell nucleus, and implicated in the regulation of chromatin and nuclear structure, as well as being involved in cytoplasmic functions. We demonstrate here that mouse ArpM1, which closely resembles the conventional actin, is expressed exclusively in the testis, particularly in haploid germ cells. ArpM1 protein first appears in the round spermatid and changes its localization dynamically in the nucleus during spermiogenesis. By co-immunoprecipitation analysis, profilin III was identified as ArpM1-interacting protein. Our findings suggest that the testis-specific profilin III–ArpM1 complex may be involved in conformational changes in the organization of the sperm-specific nucleus.

Structured summary


MINT-6741685, MINT-6741696, MINT-6741707:

Arpm1 (uniprotkb:Q8BXF8) physically interacts (MI:0218) with Profilin-3 (uniprotkb:Q9DAD6) by antibait coimmunoprecipitation (MI:0006)

MINT-6741718:

Profilin-3 (uniprotkb:Q9DAD6) and Arpm1 (uniprotkb:Q8BXF8) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

MINT-6741756:

Profilin-1 (uniprotkb:P62962) binds (MI:0407) to beta actin (uniprotkb:P60710) by pull-down (MI:0096)

MINT-6741650:

Arpm1 (uniprotkb:Q8BXF8) physically interacts (MI:0218) with C030004A17Rik (uniprotkb:Q8C569), Vcp p97/p47 (uniprotkb:Q8CDG3), Kif21a (uniprotkb:Q9QXL2), Zfp291 (uniprotkb:Q501L8), Vcp (uniprotkb:Q01853) and Profilin-3(uniprotkb:Q9DAD6) by antibait coimmunoprecipitation (MI:0006)

MINT-6741746:

Profilin-3 (uniprotkb:Q9DAD6) binds (MI:0407) to beta actin (uniprotkb:P60710) by pull-down (MI:0096)

MINT-6741735:

Abbreviations: Arp, actin-related protein, PBS, phosphate-buffered saline, PFA, paraformaldehyde, RT, room temperature, DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride, GST, glutathione S-transferase, ER, endoplasmic reticulum

Keywords: Actin-related protein, Profilin, Nuclear structure, Spermatid, Spermatogenesis, Testis

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PII: S0014-5793(08)00665-0

doi:10.1016/j.febslet.2008.07.058

FEBS Letters
Volume 582, Issue 20 , Pages 2998-3004, 3 September 2008

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