Reciprocal regulation of 12- and 15-lipoxygenases by UV-irradiation in human keratinocytes

Yoo, Hyun; Jeon, Byeongwook; Jeon, Myung-Shin; Lee, Hyangkyu; Kim, Tae-Yoon

doi:10.1016/j.febslet.2008.08.017

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Volume 582, Issue 21 , Pages 3249-3253, 22 September 2008

Reciprocal regulation of 12- and 15-lipoxygenases by UV-irradiation in human keratinocytes

Edited by Sandro Sonnino

Laboratory of Dermato-Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-040, South Korea

Received 4 August 2008; received in revised form 19 August 2008; accepted 19 August 2008. published online 26 August 2008.

Abstract

The 12/15-lipoxygenase (12/15-LOX) pathways of arachidonate metabolism have been implicated in the pathogenesis of psoriasis. Since UV photo-therapy is a commonly used technique for inhibiting cell proliferation and inflammation in skin diseases, we hypothesized that UV-irradiation may affect 12/15-LOX expression which might regulate cell proliferation. In this study, we showed that UV-irradiation suppressed 12-LOX expression, whereas up-regulated 15-LOX expression. Treatment with the 15-LOX metabolites sufficiently suppressed insulin-like growth factor II-induced 12-LOX expression and blocked cell cycle progression. On the basis of our findings, we think that the 15-LOX metabolites may inhibit epidermal hyperplasia in psoriasis by regulating 12-LOX expression.

Abbreviations: IGF-II, insulin-like growth factor II, 12/15-LOX, 12/15-lipoxygenase, PUVA, psoralen plus ultraviolet A

Keywords: 12/15-Lipoxygenase (LOX), HaCaT, IGF-II, Psoriasis, UV