The role of Mac-1 (CD11b/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-κB ligand

Hayashi, Hidetaka; Nakahama, Ken-ichi; Sato, Takahiro; Tuchiya, Takehiko; Asakawa, Yasuyuki; Maemura, Toshimitu; Tanaka, Masanobu; Morita, Mineto; Morita, Ikuo

doi:10.1016/j.febslet.2008.08.023

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Volume 582, Issue 21 , Pages 3243-3248, 22 September 2008

The role of Mac-1 (CD11b/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-κB ligand

Edited by Laszlo Nagy

Hidetaka Hayashi
- Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Ken-ichi Nakahama
- Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
- Corresponding author. Fax: +81 5803 0212.
Takahiro Sato
- Department of Natural Resources and Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
Takehiko Tuchiya
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Yasuyuki Asakawa
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Toshimitu Maemura
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Masanobu Tanaka
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Mineto Morita
- Toho University Omori Medical Center, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan
Ikuo Morita
- Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan

Received 23 June 2008; received in revised form 5 August 2008; accepted 26 August 2008. published online 03 September 2008.

Abstract

Multinuclear osteoclasts are derived from CD11b-positive mononuclear cells in bone marrow and in circulation. FACS sorting experiments showed impaired osteoclastogenesis in RAW264.7 cells with low CD11b expression. Neutralizing antibodies and siRNA against CD11b inhibited osteoclastogenesis induced by RANKL. Although primary cultured mouse bone marrow macrophages expressed CD11a and CD11b, osteoclastogenesis induced by M-CSF and RANKL was inhibited in the presence of anti-CD11b or anti-CD18 but not anti-CD11a antibodies. Furthermore, anti-CD11b antibodies inhibited NFATc1 expression induced by M-CSF and RANKL in BMMs. These findings suggest, at least partly, an important role of CD11b in osteoclastogenesis.

Keywords: Osteoclastogenesis, Nuclear factor of activated T cells c1, RAW 264.7 cell line, Receptor activator of nuclear factor-κB ligand, Integrin