FEBS Letters
Volume 582, Issue 23 , Pages 3270-3274, 15 October 2008

Juxtamembrane basic residues in glycoprotein Ibβ cytoplasmic domain are required for assembly and surface expression of glycoprotein Ib-IX complex

Edited by Gianni Cesareni

  • Xi Mo

      Affiliations

    • Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    • ,
  • Shi-Zhong Luo

      Affiliations

    • Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    • ,
  • José A. López

      Affiliations

    • Puget Sound Blood Center, Division of Hematology, Department of Medicine, University of Washington, Seattle, WA 98104, USA
    • ,
  • Renhao Li

      Affiliations

    • Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    • Corresponding Author InformationCorresponding author. Fax: +1 713 500 0545.

Received 28 May 2008; received in revised form 3 August 2008; accepted 6 August 2008. published online 10 September 2008.

Abstract 

Platelet glycoprotein (GP) Ib-IX complex requires all its three subunits for efficient expression on the cell surface, but the underlying molecular basis is not fully clear. Using transfected Chinese hamster ovary cells as the model system, we demonstrate that juxtamembrane residues 149–154 in the cytoplasmic domain of the GPIbβ subunit is required for assembly and surface expression of the GPIb-IX complex. The complex, or GPIbβ by itself, lacking these residues is retained in the endoplasmic reticulum. Our results thus have illustrated an important role of the GPIbβ cytoplasmic domain in biosynthesis of the GPIb-IX complex.

Structured summary


MINT-6742751, MINT-6742907:

GPIX (uniprotkb:P14770), GPIbalpha (uniprotkb:P07359)

and GPIbbeta (uniprotkb:P13224) physically interact (MI:0218) by fluorescence-activated cell sorting (MI:0054)

Abbreviations: GP, glycoprotein, CHO, Chinese hamster ovary, ER, endoplasmic reticulum

Keywords: GPIb-IX complex, Juxtamembrane region, Polybasic residue cluster, Protein biosynthesis, Trafficking

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PII: S0014-5793(08)00729-1

doi:10.1016/j.febslet.2008.08.036

FEBS Letters
Volume 582, Issue 23 , Pages 3270-3274, 15 October 2008

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