FEBS Letters
Volume 582, Issue 23 , Pages 3281-3287, 15 October 2008

Interaction of grape ASR proteins with a DREB transcription factor in the nucleus

Edited by Ulf-Ingo Flügge

  • Amélie Saumonneau

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Alice Agasse

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Marie-Thérèse Bidoyen

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Magali Lallemand

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Anne Cantereau

      Affiliations

    • Service commun de microscopie confocale, Institut de Physiologie et Biologie Cellulaire (IPBC), UMR CNRS 6187, Université de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Anna Medici

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Maryse Laloi

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
  • ,
  • Rossitza Atanassova

      Affiliations

    • Université de Poitiers, FRE CNRS 3091 “Physiologie moléculaire du transport des sucres”, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
    • Corresponding Author InformationCorresponding author. Fax: +33 (0) 549454186.

Received 23 July 2008; received in revised form 1 September 2008; accepted 2 September 2008. published online 18 September 2008.

Abstract 

ASR proteins (abscissic acid, stress, ripening induced) are involved in plant responses to developmental and environmental signals but their biological functions remain to be elucidated. Grape ASR gene (VvMSA) encodes a new transcription factor regulating the expression of a glucose transporter. Here, we provide evidence for some polymorphism of grape ASRs and their identification as chromosomal non-histone proteins. By the yeast two-hybrid approach, a protein partner of VvMSA is isolated and characterized as an APETALA2 domain transcription factor. Interaction of the two proteins is further demonstrated by the BiFC approach and the exclusive nuclear localization of the heterodimer is visualized.

Structured summary


MINT-6743067: VvMSA (uniprotkb:Q94G23) and VvDREB (uniprotkb:A6XA90) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809)

MINT-6743043: VvMSA (uniprotkb:Q94G23) physically interacts (MI:0218) with VvDREB (uniprotkb:A6XA90) by two hybrid (MI:0018)

Keywords: ASR proteins, DREB transcription factor, Protein-protein interaction, Yeast two-hybrid, BiFC, Grapevine

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PII: S0014-5793(08)00748-5

doi:10.1016/j.febslet.2008.09.015

FEBS Letters
Volume 582, Issue 23 , Pages 3281-3287, 15 October 2008