d-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1

Grigston, Jeffrey C.; Osuna, Daniel; Scheible, Wolf-Rüdiger; Liu, Chenggang; Stitt, Mark; Jones, Alan M.

doi:10.1016/j.febslet.2008.08.038

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Volume 582, Issue 25 , Pages 3577-3584, 29 October 2008

d-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1

Edited by Micheal R. Sussman

Jeffrey C. Grigston
- Department of Biology, CB#3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA
Daniel Osuna
- Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Wolf-Rüdiger Scheible
- Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Chenggang Liu
- Department of Biology, CB#3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA
Mark Stitt
- Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Alan M. Jones
- Department of Biology, CB#3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA
- Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
- Corresponding author. Address: Department of Biology, CB#3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA. Fax: +1 919 962 1625.

Received 11 July 2008; received in revised form 22 August 2008; accepted 25 August 2008. published online 23 September 2008.

Abstract

Plants use sugars as signaling molecules and possess mechanisms to detect and respond to changes in sugar availability, ranging from the level of secondary signaling molecules to altered gene transcription. G-protein-coupled pathways are involved in sugar signaling in plants. The Arabidopsis thaliana regulator of G-protein signaling protein 1 (AtRGS1) combines a receptor-like seven transmembrane domain with an RGS domain, interacts with the Arabidopsis Gα subunit (AtGPA1) in a d-glucose-regulated manner, and stimulates AtGPA1 GTPase activity. We determined that AtRGS1 interacts with additional components, genetically defined here, to serve as a plasma membrane sensor for d-glucose. This interaction between AtRGS1 and AtGPA1 involves, in part, the seven-transmembrane domain of AtRGS1.