Taxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae

Ahmed, Neesar; Dasari, Sreekanth; Srivastava, Saumya S.; Sneh, Amita; Ahmad, Absar; Islam Khan, M.; Krishnasastry, M.V.

doi:10.1016/j.febslet.2008.09.029

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Volume 582, Issue 25 , Pages 3595-3600, 29 October 2008

Taxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae

Edited by Lukas Huber

Neesar Ahmed
- National Centre for Cell Science, Ganeshkhind Road, University of Pune Campus, Pune 411 007, India
Sreekanth Dasari
- National Chemical Laboratory, Pashan Road, Pune 411 007, India
Saumya S. Srivastava
- National Centre for Cell Science, Ganeshkhind Road, University of Pune Campus, Pune 411 007, India
- Authors made equal contribution.
Amita Sneh
- National Centre for Cell Science, Ganeshkhind Road, University of Pune Campus, Pune 411 007, India
- Authors made equal contribution.
Absar Ahmad
- National Chemical Laboratory, Pashan Road, Pune 411 007, India
M. Islam Khan
- National Chemical Laboratory, Pashan Road, Pune 411 007, India
M.V. Krishnasastry
- National Centre for Cell Science, Ganeshkhind Road, University of Pune Campus, Pune 411 007, India
- Corresponding author. Fax: +91 20 25692259.

Received 27 July 2008; received in revised form 12 September 2008; accepted 15 September 2008. published online 23 September 2008.

Abstract

Taxol treatment of HeLa cells resulted in a transient recruitment of Caveolin-1 to the cell surface followed by internalization. Interestingly, 20min after 10-deacetylbaccatinIII (10-DAB) treatment, the caveolae displayed faster ‘kiss and run’ dynamics while BaccatinIII (BacIII) did not induce any change. Sustained phosphorylation of Caveolin-1 is observed upon treatment and between Taxol and 10-DAB, the former shows phosphorylated Raf-1, ERK1/2 and hyperphosphorylated Bcl-2 while the later showed much less magnitude of the same. BacIII treatment did not induce phosphorylation of Raf-1 or Bcl-2. It is possible that Taxol might act on multiple targets and the side chain may be crucial.