Lipid modulation of membrane-bound epitope recognition and blocking by HIV-1 neutralizing antibodies

Abstract 

The conserved, aromatic-rich membrane-proximal external region (MPER) of gp41 is functional in human immunodeficiency virus (HIV)-cell fusion by perturbing membrane integrity. Broadly-neutralizing 2F5 and 4E10 monoclonal antibodies (MAb-s) recognize amino- and carboxy-terminal epitope sequences within this domain, respectively. An MPER peptide overlapping 2F5 and 4E10 epitope sequences was capable of breaching the permeability barrier of lipid vesicles. Cholesterol and sphingomyelin raft-lipids, present at high quantities in the HIV-1 envelope, promoted exposure or occlusion of 4E10 epitope, respectively. Conversely, 2F5 epitope accessibility was affected to a lesser extent by these envelope lipids. These observations support the idea that MPER epitopes on membranes are segmented in terms of how they are affected by envelope lipids, which may have implications for MPER-based vaccine development.

Abbreviations: Chol, cholesterol, CRAC, Cholesterol Recognition/interaction Amino acid Consensus motif, DPC, dodecylphosphocholine, HIV-1, human immunodeficiency virus type-1, MAb, monoclonal antibody, MPER, membrane-proximal external region, NAb-s, neutralizing antibodies, POPC, phosphatidylcholine, PL, phospholipid, PreTM, pretransmembrane, SPM, sphingomyelin, WW, Wimley–White

Keywords: HIV-1, HIV-1 gp41, HIV-1 neutralization, MAb2F5, MAb4E10, MPER, Cholesterol, Sphingomyelin