Subunit stoichiometry of the Drosophila melanogaster small nuclear RNA activating protein complex (SNAPc)

Lai, Hsien-Tsung; Kang, Yoon Soon; Stumph, William E.

doi:10.1016/j.febslet.2008.09.059

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Volume 582, Issue 27 , Pages 3734-3738, 12 November 2008

Subunit stoichiometry of the Drosophila melanogaster small nuclear RNA activating protein complex (SNAPc)

Edited by Lukas Huber

Hsien-Tsung Lai
- Department of Biology, San Diego State University, San Diego, CA 92182-1030, United States
- Molecular Biology Institute, San Diego State University, San Diego, CA 92182-1030, United States
Yoon Soon Kang
- Molecular Biology Institute, San Diego State University, San Diego, CA 92182-1030, United States
- Department of Chemistry and Biochemistry, San Diego State University, San Diego, CA 92182-1030, United States
William E. Stumph
- Molecular Biology Institute, San Diego State University, San Diego, CA 92182-1030, United States
- Department of Chemistry and Biochemistry, San Diego State University, San Diego, CA 92182-1030, United States
- Corresponding author. Address: Department of Chemistry and Biochemistry, San Diego State University, San Diego, CA 92182-1030, United States. Fax: +1 619 594 4634.

Received 4 August 2008; received in revised form 22 September 2008; accepted 27 September 2008. published online 22 October 2008.

Abstract

Small nuclear RNA activating protein complex (SNAPc) is a multi-subunit transcription factor required for expression of small nuclear RNA genes. This protein binds to a promoter element located approximately 40–65bp upstream of the transcription start site. In Drosophila melanogaster, DmSNAPc contains three distinct polypeptide subunits: DmSNAP190, DmSNAP50, and DmSNAP43. The subunit stoichiometry in SNAPc complexed with DNA has not been examined. Therefore, the ability of differently tagged but otherwise identical subunits to associate with each other into the same protein–DNA complex was assayed by antibody super-shift analysis. The results reveal that DmSNAPc contains only a single copy of each of the three subunits.

Structured summary

MINT-6788068:

DmSNAP43 (uniprotkb:Q9VF25), DmSNAP50 (uniprotkb:Q7JUY8) and DmSNAP190 (uniprotkb:Q9VHX0) physically interact (MI:0218) by electrophoretic mobility shift assay (MI:0413)

MINT-6788151:

DmSNAP190 (uniprotkb:Q9VHX0), DmSNAP50 (uniprotkb:Q7JUY8) and DmSNAP43 (uniprotkb:Q9VF25) physically interact (MI:0218) by molecular sieving (MI:0071)