Biphasic effect of insulin on beta cell apoptosis depending on glucose deprivation

Guillen, C.; Bartolomé, A.; Nevado, C.; Benito, M.

doi:10.1016/j.febslet.2008.10.020

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Volume 582, Issue 28 , Pages 3855-3860, 26 November 2008

Biphasic effect of insulin on beta cell apoptosis depending on glucose deprivation

Edited by Robert Barouki

C. Guillen
- Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain
- CIBER de Diabetes y Enfermedades Metabólicas Asociadas, ISCIII, Spain
A. Bartolomé
- Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain
- CIBER de Diabetes y Enfermedades Metabólicas Asociadas, ISCIII, Spain
C. Nevado
- Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain
M. Benito
- Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain
- CIBER de Diabetes y Enfermedades Metabólicas Asociadas, ISCIII, Spain
- Corresponding author. Present address: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain.

Received 18 September 2008; received in revised form 6 October 2008; accepted 13 October 2008. published online 22 October 2008.

Abstract

Insulin resistant states are associated with an increase in the beta cell mass and also high levels of circulating insulin. Ultimately the beta cells undergo a failure that leads to diabetes. At this stage, a question arises if those persistent high levels of circulating insulin may contribute to beta cell damage. To address this important issue, we submitted beta cells to a prolonged effect of increasing concentrations of insulin. We observed that a prolonged effect of high levels of insulin on the presence of serum (15–24h) in glucose-deprived beta cells induced apoptosis. This apoptotic effect was both dose- and cycloheximide-dependent.