Regulation of the human PDZK1 expression by peroxisome proliferator-activated receptor alpha

Tachibana, Keisuke; Anzai, Naohiko; Ueda, Chihiro; Katayama, Tatsuya; Yamasaki, Daisuke; Kirino, Takayoshi; Takahashi, Rika; Ishimoto, Kenji; Komori, Hidenori; Tanaka, Toshiya; Hamakubo, Takao; Ueda, Yukihiko; Arai, Hiroyuki; Sakai, Juro; Kodama, Tatsuhiko; Doi, Takefumi

doi:10.1016/j.febslet.2008.10.027

« Previous Next »FEBS Letters
Volume 582, Issue 28 , Pages 3884-3888, 26 November 2008

Regulation of the human PDZK1 expression by peroxisome proliferator-activated receptor alpha

Edited by Ivan Sadowski

Keisuke Tachibana
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
Naohiko Anzai
- Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan
Chihiro Ueda
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
Tatsuya Katayama
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
Daisuke Yamasaki
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
- The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Takayoshi Kirino
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
Rika Takahashi
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
Kenji Ishimoto
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
- Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Hidenori Komori
- Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
Toshiya Tanaka
- Laboratory for System Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan
Takao Hamakubo
- Laboratory for System Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan
Yukihiko Ueda
- Hirakata Kohsai Hospital, 1-2-1 Fujisaka-Higashi-machi, Hirakata, Osaka 573-0153, Japan
Hiroyuki Arai
- Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan
Juro Sakai
- Laboratory for System Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan
Tatsuhiko Kodama
- Laboratory for System Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan
Takefumi Doi
- Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
- The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- Corresponding author. Address: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. Fax: +81 6 6879 8158.

Received 11 September 2008; received in revised form 30 September 2008; accepted 3 October 2008. published online 27 October 2008.

Abstract

Although PDZK1 is a well-known adaptor protein, the mechanisms for its role in transcriptional regulation are largely unknown. The peroxisome proliferator-activated receptor alpha (PPARα) is a ligand-activated transcription factor that plays an important role in the regulation of lipid homeostasis. Previously, we established a tetracycline-regulated human cell line that can be induced to express PPARα and identified candidate target genes, one of which was PDZK1. In this study, we cloned and characterized the promoter region of the human pdzk1 gene and determined the PPAR response element. Finally, we demonstrate that endogenous PPARα regulates PDZK1 expression.

Abbreviations: PPAR, peroxisome proliferator-activated receptor, RXR, retinoid X receptor, PPRE, peroxisome proliferator responsive element

Keywords: PPARα, PDZK1, PPRE, Target gene