FEBS Letters
Volume 582, Issue 28 , Pages 3903-3908, 26 November 2008

Specific interaction of TFII-I with an upstream element on the HIV-1 LTR regulates induction of latent provirus

Edited by Aleksander Benjak

Department of Biochemistry and Molecular Biology, Molecular Epigenetics, LSI, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3

Received 25 April 2008; received in revised form 19 September 2008; accepted 16 October 2008. published online 28 October 2008.

Abstract 

RBF-2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV-1 LTR requires a co-factor TFII-I. We have identified specific nucleotides, immediately 3′ of RBEIII that are required for stable association of TFII-I with this region of the LTR. Mutations that inhibit interaction of TFII-I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII-I bound at an upstream LTR element for viral replication.

Keywords: HIV-1, TFII-I, USF, RBF-2, MAPK signaling

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PII: S0014-5793(08)00850-8

doi:10.1016/j.febslet.2008.10.032

FEBS Letters
Volume 582, Issue 28 , Pages 3903-3908, 26 November 2008

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