Crystal structure of mSULT1D1, a mouse catecholamine sulfotransferase

Teramoto, Takamasa; Sakakibara, Yoichi; Inada, Kanako; Kurogi, Katsuhisa; Liu, Ming-Cheh; Suiko, Masahito; Kimura, Makoto; Kakuta, Yoshimitsu

doi:10.1016/j.febslet.2008.10.035

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Volume 582, Issue 28 , Pages 3909-3914, 26 November 2008

Crystal structure of mSULT1D1, a mouse catecholamine sulfotransferase

Edited by Peter Brzezinski

Takamasa Teramoto
- Laboratory of Structural Biology, Department of Systems Life Sciences, Japan
Yoichi Sakakibara
- Food Research Branch, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
Kanako Inada
- Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
Katsuhisa Kurogi
- Food Research Branch, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
Ming-Cheh Liu
- Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43606, USA
Masahito Suiko
- Food Research Branch, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
Makoto Kimura
- Laboratory of Structural Biology, Department of Systems Life Sciences, Japan
- Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
Yoshimitsu Kakuta
- Laboratory of Structural Biology, Department of Systems Life Sciences, Japan
- Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
- Corresponding author. Address: Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. Fax: +81 92 642 2854.

Received 11 August 2008; received in revised form 21 October 2008; accepted 21 October 2008. published online 31 October 2008.

Abstract

In mammals, sulfonation as mediated by specific cytosolic sulfotransferases (SULTs) plays an important role in the homeostasis of dopamine and other catecholamines. To gain insight into the structural basis for dopamine recognition/binding, we determined the crystal structure of a mouse dopamine-sulfating SULT, mouse SULT1D1 (mSULT1D1). Data obtained indicated that mSULT1D1 comprises of a single α/β domain with a five-stranded parallel β-sheet. In contrast to the structure of the human SULT1A3 (hSULT1A3)-dopamine complex previously reported, molecular modeling and mutational analysis revealed that a water molecule plays a critical role in the recognition of the amine group of dopamine by mSULT1D1. These results imply differences in substrate binding between dopamine-sulfating SULTs from different species.

Keywords: Sulfotransferase, Crystal structure, SULT1D1, Catecholamine metabolism, Substrate recognition