FEBS Letters
Volume 582, Issue 29 , Pages 3967-3972, 10 December 2008

The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis

Edited by Maurice Montal

  • Christian Klenner

      Affiliations

    • Institute of Microbiology and Molecular Biology, University of Hohenheim, 70599 Stuttgart, Germany
    • ,
  • Jijun Yuan

      Affiliations

    • Ohio State University, Department of Chemistry, 100 West 18th Avenue, Columbus, OH 43210, USA
    • ,
  • Ross E. Dalbey

      Affiliations

    • Ohio State University, Department of Chemistry, 100 West 18th Avenue, Columbus, OH 43210, USA
    • ,
  • Andreas Kuhn

      Affiliations

    • Institute of Microbiology and Molecular Biology, University of Hohenheim, 70599 Stuttgart, Germany
    • Corresponding Author InformationCorresponding author. Fax: +49 711 459 22238.

Received 19 September 2008; received in revised form 8 October 2008; accepted 10 October 2008. published online 07 November 2008.

Abstract 

The coat protein of bacteriophage Pf3 is inserted into the plasma membrane of Escherichia coli by the insertase YidC. To identify which of the six transmembrane regions of YidC bind the single-spanning Pf3 coat protein during membrane protein biogenesis, we used the disulfide cross-linking approach. We generated single cysteines in each of the transmembrane regions of YidC and in the center of the hydrophobic region of Pf3 coat protein. We found that the substrate Pf3 coat contacts the first and third transmembrane segment (TM) of YidC as crosslinks between these two proteins can be formed in vivo during membrane biogenesis. A detailed disulfide-mapping study revealed that one face of TM3 of YidC makes contact with the Pf3 protein.

Structured summary


MINT-6795850, MINT-6795869, MINT-6795912, MINT-6795927, MINT-6795942:

Coat protein (uniprotkb:P03623) binds (MI:0408) YidC (uniprotkb:P25714) by cross-linking studies (MI:0030)

MINT-6795898:

Coat protein (uniprotkb:P03623) binds (MI:0408) Coat protein (uniprotkb:P03623) by cross-linking studies (MI:0030)

Keywords: Membrane insertase, Disulfide mapping, Translocase, Transmembrane helix, Membrane biogenesis, Helix–helix contact

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PII: S0014-5793(08)00876-4

doi:10.1016/j.febslet.2008.10.044

FEBS Letters
Volume 582, Issue 29 , Pages 3967-3972, 10 December 2008

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