Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae

Abstract 

A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway.

Abbreviations: DMAPP, dimethyl allyl diphosphate, G3P, glyceraldehyde 3-phosphate, GC–MS, gas chromatography–mass spectrometry, IPP, isopentenyl diphosphate, MEP, 2-C-methyl-d-erythritol 4-phosphate, MVA, mevalonate, RT-PCR, reverse transcriptase polymerase chain reaction

Keywords: Isoprenoid, Yeast, 2-Methyl erythritol 4-phosphate, Mevalonate, Saccharomyces cerevisiae