Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP)

El Bounkari, Omar; Guria, Anuja; Klebba-Faerber, Sabine; Claußen, Maike; Pieler, Tomas; Griffiths, John R.; Whetton, Anthony D.; Koch, Alexandra; Tamura, Teruko

doi:10.1016/j.febslet.2008.11.024

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Volume 583, Issue 1 , Pages 13-18, 5 January 2009

Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP)

Edited by Ulrike Kutay

Omar El Bounkari
- Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany
Anuja Guria
- Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany
Sabine Klebba-Faerber
- Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany
Maike Claußen
- Georg-August Universität Göttingen, Zentrum für Biochemie und Molekular Zellbiologie, Abteilung Entwicklungsbiochemie, Justus-von-Liebig Weg 11, D-37077 Göttingen, Germany
Tomas Pieler
- Georg-August Universität Göttingen, Zentrum für Biochemie und Molekular Zellbiologie, Abteilung Entwicklungsbiochemie, Justus-von-Liebig Weg 11, D-37077 Göttingen, Germany
John R. Griffiths
- School of Cancer and Imaging science, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK
Anthony D. Whetton
- School of Cancer and Imaging science, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK
Alexandra Koch
- Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany
Teruko Tamura
- Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany
- Corresponding author. Fax: +49 511 532 2827.

Received 1 September 2008; received in revised form 6 November 2008; accepted 7 November 2008. published online 05 December 2008.

Abstract

THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50–137, amino acid numbers) binds to the N-terminal portion (1–199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.

Structured summary

MINT-6799962, MINT-6799973, MINT-6800005: THOC7 (uniprotkb:Q6I9Y2) physically interacts (MI:0218) with THOC5 (uniprotkb:Q13769) by pull down (MI:0096)

MINT-6800108: FMIP (uniprotkb:Q13769) and THOC7 (uniprotkb: Q6I9Y2) co-localize (MI:0403) by fluorescence microscopy (MI:0416)

MINT-6800052: FMIP (uniprotkb:Q13769) physically interacts (MI:0218) with THOC1 (uniprotkb: Q96FV9) by anti tag coimmunoprecipitation (MI:0007)

MINT-6800022: THOC7 (uniprotkb:Q6I9Y2) physically interacts (MI:0218) with FMIP (uniprotkb:Q6DFL5) by pull down (MI:0096)

MINT-6799989: THOC7 (uniprotkb:Q6I9Y2) binds (MI:0407) to FMIP (uniprotkb:Q13769) by pull down (MI:0096)

MINT-6800071, MINT-6800089: FMIP (uniprotkb:Q13769) physically interacts (MI:0218) with THOC7 (uniprotkb:Q6I9Y2) and THOC1 (uniprotkb:Q96FV9) by anti tag coimmunoprecipitation (MI:0007)