O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

Lim, Kihong; Chang, Hyo-Ihl

doi:10.1016/j.febslet.2008.12.007

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Volume 583, Issue 3 , Pages 512-520, 4 February 2009

O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

Edited by Ivan Sadowski

School of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea

Received 27 October 2008; received in revised form 25 November 2008; accepted 2 December 2008. published online 12 December 2008.

Abstract

Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity. Yet, how O-GlcNAc on other modification sites affects Sp1 function and how O-GlcNAcylation of Sp1 affects the transcriptional regulation of a target gene remains unknown. Here we show that O-GlcNAc within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1, and inhibits the cooperative activation of the U2 snRNA gene by Sp1 and Oct1.

Structured summary

MINT-6803452: Sp1 (uniprotkb-P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti tag coimmunoprecipitation (MI:0007)

MINT-6803426, MINT-6803438: Oct1 (uniprotkb:P14859) binds (MI:0407) to Sp1 (uniprotkb:P08047) by pull down (MI:0096)

MINT-6803470, MINT-6803484: Sp1 (uniprotkb:P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti bait coimmunoprecipitation (MI:0006)

Keywords: O-GlcNAc, Oct1, Sp1, U2 snRNA