FEBS Letters
Volume 583, Issue 2 , Pages 266-270, 22 January 2009

Distinct gate conformations of the ABC transporter BtuCD revealed by electron spin resonance spectroscopy and chemical cross-linking

Edited by Peter Brzezinski

  • Birke A. Goetz

      Affiliations

    • Institute of Molecular Biology and Biophysics, ETH Zurich, HPK D14.3, 8093 Zurich, Switzerland
    • ,
  • Eduardo Perozo

      Affiliations

    • Institute of Molecular Pediatric Science and Department of Biochemistry and Molecular Biology, University of Chicago, 929 E 57th St., CIS W206, Chicago, IL 60637, USA
    • ,
  • Kaspar P. Locher

      Affiliations

    • Institute of Molecular Biology and Biophysics, ETH Zurich, HPK D14.3, 8093 Zurich, Switzerland
    • Corresponding Author InformationCorresponding author.

Received 20 October 2008; received in revised form 8 December 2008; accepted 9 December 2008. published online 19 December 2008.

Abstract 

BtuCD is a type II ABC importer that catalyzes the translocation of vitamin B12 from the periplasm into the cytoplasm of Escherichia coli. Crystal structures of BtuCD and the related HiF (or Hi1470/71) protein from Haemophilus influenzae have revealed distinct conformations of the transmembrane domains that form inner and outer gates. We used electron spin resonance spectroscopy to study the reaction cycle of BtuCD after labeling the protein at residues located at these gates. The results suggest that BtuCD as a prototype type II ABC importer may have a mechanism that is distinct from that of ABC exporters such as Sav1866 or type I ABC importers such as those specific for molybdate (ModBC) or maltose (MalFGK).

Structured summary

MINT-6803800: btuF (uniprotkb: P37028), btuC (uniprotkb:P06609) and btuD (uniprotkb:P06611) physically interact (MI:0218) by molecular sieving (MI:0071)

Keywords: ABC transporter, Transport mechanism, Electron paramagnetic resonance (EPR) spectroscopy, Crosslinking

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PII: S0014-5793(08)01001-6

doi:10.1016/j.febslet.2008.12.020

FEBS Letters
Volume 583, Issue 2 , Pages 266-270, 22 January 2009

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