Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro-binding assay in a butterfly

Tsuchihara, Kazuko; Wazawa, Tetsuichi; Ishii, Yoshiharu; Yanagida, Toshio; Nishida, Ritsuo; Zheng, Xiang-Guo; Ishiguro, Masaji; Yoshihara, Kazuo; Hisatomi, Osamu; Tokunaga, Fumio

doi:10.1016/j.febslet.2008.12.027

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Volume 583, Issue 2 , Pages 345-349, 22 January 2009

Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro-binding assay in a butterfly

Edited by Gianni Cesareni

Kazuko Tsuchihara
- Iwaki Meisei University, Faculty of Pharmacy, 5-5-1 Iino, Chuo-dai, Iwaki, Fukushima 970-8551, Japan
- Department of Biological Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
- Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
- Corresponding author. Address: Iwaki Meisei University, Faculty of Pharmacy, 5-5-1 Iino, Chuo-dai, Iwaki, Fukushima 970-8551, Japan. Fax: +81 246 29 5414.
Tetsuichi Wazawa
- Single Molecule Processes Project, International Cooperative Research Project, Minoo, Osaka 562-0035, Japan
Yoshiharu Ishii
- Single Molecule Processes Project, International Cooperative Research Project, Minoo, Osaka 562-0035, Japan
Toshio Yanagida
- Single Molecule Processes Project, International Cooperative Research Project, Minoo, Osaka 562-0035, Japan
Ritsuo Nishida
- Laboratory of Chemical Ecology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
Xiang-Guo Zheng
- Suntory Institute for Bioorganic Research, Shimamoto-cho, Osaka 618-8503, Japan
Masaji Ishiguro
- Suntory Institute for Bioorganic Research, Shimamoto-cho, Osaka 618-8503, Japan
Kazuo Yoshihara
- Suntory Institute for Bioorganic Research, Shimamoto-cho, Osaka 618-8503, Japan
Osamu Hisatomi
- Department of Biological Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
- Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
Fumio Tokunaga
- Department of Biological Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
- Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan

Received 4 October 2008; received in revised form 8 December 2008; accepted 9 December 2008. published online 19 December 2008.

Abstract

A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated previously from the chemoreceptive organs was assumed to bind to an oviposition stimulant. Using a highly sensitive fluorescent micro-binding assay, we clarified OSBP bound to aristolochic acid. Three-dimensional molecular modeling revealed the structure of the OSBP-aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant as a ligand in insects.

Keywords: Binding assay, Chemosensory reception, Oviposition stimulant, Binding protein, Molecular modeling