FEBS Letters
Volume 583, Issue 3 , Pages 549-555, 4 February 2009

Individual and common inhibitors of coronavirus and picornavirus main proteases

Edited by Hans-Dieter Klenk

  • Chih-Jung Kuo

      Affiliations

    • Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC
    • Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan
    • ,
  • Hun-Ge Liu

      Affiliations

    • Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC
    • ,
  • Yueh-Kuei Lo

      Affiliations

    • Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC
    • ,
  • Churl-Min Seong

      Affiliations

    • Korea Research Institute of Chemical Technology, Daejeon 305-606, Republic of Korea
    • ,
  • Kee-In Lee

      Affiliations

    • Korea Research Institute of Chemical Technology, Daejeon 305-606, Republic of Korea
    • ,
  • Young-Sik Jung

      Affiliations

    • Korea Research Institute of Chemical Technology, Daejeon 305-606, Republic of Korea
    • Corresponding Author InformationCorresponding authors. Address: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC. Fax: +886 2 2788 9759.
    • ,
  • Po-Huang Liang

      Affiliations

    • Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC
    • Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan
    • Corresponding Author InformationCorresponding authors. Address: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC. Fax: +886 2 2788 9759.

Received 5 November 2008; received in revised form 17 December 2008; accepted 23 December 2008. published online 21 January 2009.

Abstract 

Picornaviruses (PV) and coronaviruses (CoV) are positive-stranded RNA viruses which infect millions of people worldwide each year, resulting in a wide range of clinical outcomes. As reported in this study, using high throughput screening against ∼6800 small molecules, we have identified several novel inhibitors of SARS-CoV 3CLpro with IC50 of low μM. Interestingly, one of them equally inhibited both 3Cpro and 3CLpro from PV and CoV, respectively. Using computer modeling, the structural features of these compounds as individual and common protease inhibitors were elucidated to enhance our knowledge for developing anti-viral agents against PV and CoV.

Keywords: Coronavirus, Picornavirus, 3C protease, Fluorescence assay, High throughput screening, Computer modeling

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PII: S0014-5793(09)00016-7

doi:10.1016/j.febslet.2008.12.059

Refers to corrigendum:

  • Corrigendum to “Individual and common inhibitors of coronavirus and picornavirus main proteases” [FEBS Lett. 583 (2009) 549–555] , 12 May 2009

    Chih-Jung Kuo, Hun-Ge Liu, Yueh-Kuei Lo, Churl-Min Seong, Kee-In Lee, Young-Sik Jung, Po-Huang Liang
    FEBS Letters 18 June 2009 (Vol. 583, Issue 12, Page 2154)

FEBS Letters
Volume 583, Issue 3 , Pages 549-555, 4 February 2009

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