Increased -1 ribosomal frameshifting efficiency by yeast prion-like phenotype [PSI+]

Park, Hee Jung; Park, So Jung; Oh, Doo-Byoung; Lee, Sangho; Kim, Yang-Gyun

doi:10.1016/j.febslet.2009.01.015

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Volume 583, Issue 4 , Pages 665-669, 18 February 2009

Increased -1 ribosomal frameshifting efficiency by yeast prion-like phenotype [PSI+]

Edited by Michael Ibba

Hee Jung Park
- Department of Chemistry, School of Natural Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea
So Jung Park
- Department of Chemistry, School of Natural Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea
Doo-Byoung Oh
- Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea
Sangho Lee
- Department of Biological Science, School of Natural Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea
- Corresponding authors. Fax: +82 31 290 7015 (S. Lee), +82 31 290 4574 (Y.G. Kim).
Yang-Gyun Kim
- Department of Chemistry, School of Natural Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea
- Corresponding authors. Fax: +82 31 290 7015 (S. Lee), +82 31 290 4574 (Y.G. Kim).

Received 6 November 2008; received in revised form 29 December 2008; accepted 7 January 2009. published online 21 January 2009.

Abstract

Programmed -1 ribosomal frameshifting (-1RF) is usually regulated by a slippery sequence and an RNA secondary structure but can be affected by the slippery sequence combined with translational perturbation. Dramatic increases of -1RF efficiencies arise from the slippery sequences in [PSI+], an epigenetic modifier of translation termination fidelity. Curing of [PSI+] abolished such increases of -1RF efficiency. Enhanced -1RF frequency at the slippery sequences could be another physiological effect induced directly or indirectly by the perturbation of the translation process in [PSI+] cells.

Abbreviations: -1RF, -1 ribosomal frameshifting, GuHCl, guanine hydrochloride

Keywords: -1 Ribosomal frameshifting, Yeast prion, Slippery sequence, [PSI+]