Proteomic and genomic analysis of PITX2 interacting and regulating networks

Huang, Yue; Huang, Kan; Boskovic, Goran; Dementieva, Yulia; Denvir, James; Primerano, Donald A.; Zhu, Guo-Zhang

doi:10.1016/j.febslet.2009.01.028

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Volume 583, Issue 4 , Pages 638-642, 18 February 2009

Proteomic and genomic analysis of PITX2 interacting and regulating networks

Edited by Lukas Huber

Yue Huang
- Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA
Kan Huang
- Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA
Goran Boskovic
- Department of Biochemistry and Microbiology, Marshall University, Huntington, WV 25755, USA
Yulia Dementieva
- Department of Mathematics, Marshall University, Huntington, WV 25755, USA
James Denvir
- Department of Statistics, West Virginia University, Morgantown, WV 26506, USA
Donald A. Primerano
- Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA
- Department of Biochemistry and Microbiology, Marshall University, Huntington, WV 25755, USA
Guo-Zhang Zhu
- Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA
- Department of Biological Sciences, One John Marshall Drive, Marshall University, Huntington, WV 25755, USA
- Cell Differentiation and Development Center, Marshall University, Huntington, WV 25755, USA
- Corresponding author. Address: Department of Biological Sciences, One John Marshall Drive, Marshall University, Huntington, WV 25755, USA. Fax: +1 304 696 7136.

Received 18 October 2008; received in revised form 20 December 2008; accepted 15 January 2009. published online 27 January 2009.

Abstract

Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in mass spectrometry analysis. We also found that overexpression of PITX2 upregulated 868 genes (2–25-fold) and downregulated 191 genes (2–15-fold) in DNA microarray analysis. These data provide an insightful perspective for further studying PITX2 function and mechanism of action.

Structured summary

MINT-6823857: PITX2 (uniprotkb: Q99697) physically interacts (MI:0218) with hnRNP K (uniprotkb:P61978), YB-1 (uniprotkb:P67809), Nucleolin (uniprotkb:P19338) and hnRNP U (uniprotkb:Q00839) by anti tag coimmunoprecipitation (MI:0007)

MINT-6823877: PITX2 (uniprotkb:Q99697) physically interacts (MI:0218) with beta catenin (uniprotkb:P35222), hnRNP U (uniprotkb:Q00839), Nucleolin (uniprotkb:P19338), YB-1 (uniprotkb:P67809) and hnRNP K (uniprotkb:P61978) by anti tag coimmunoprecipitation (MI:0007)

MINT-6823902: YB-1 (uniprotkb:P67809) physically interacts (MI:0218) with PITX2 (uniprotkb: Q99697) by anti bait coimmunoprecipitation (MI:0006)

Abbreviations: PITX2, pituitary homeobox 2, YB-1, Y box binding factor-1, hnRNP U, heterogeneous nuclear ribonucleoprotein U, hnRNP K, heterogeneous ribonucleoprotein K, NF-1, nuclear factor-1, HMG-17, high mobility group-17, GcMa, glial cells missing a, PLOD, procollagen lysyl hydroxylase