FEBS Letters
Volume 583, Issue 4 , Pages 754-758, 18 February 2009

Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids

Edited by Julian Schroeder

  • Stephen H. Loukin

      Affiliations

    • Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive Madison, WI 53706, United States
    • ,
  • Zhenwei Su

      Affiliations

    • Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive Madison, WI 53706, United States
    • ,
  • Ching Kung

      Affiliations

    • Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive Madison, WI 53706, United States
    • Department of Genetics, University of Wisconsin-Madison, Madison, WI53706, United States
    • Corresponding Author InformationCorresponding author. Address: Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive Madison, WI 53706, United States.

Received 14 November 2008; received in revised form 14 January 2009; accepted 16 January 2009. published online 27 January 2009.

Abstract 

Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca2+ rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks activate TRPV4 but not TRPV1. Osmotic swelling is modeled to activate enzyme(s), producing polyunsaturated fatty acids (PUFAs) to open TRPV4 in mammalian cells. This model relegates mechanosensitivity to the enzyme and not the channel. Yeast has only a single Δ9 fatty-acid monodesaturase and cannot make PUFAs suggesting an alternative mechanism for TRPV4 activation. We discuss possible explanations of this difference.

Keywords: Transient-receptor-potential channel, Mechanosensitivity, Thermosensitivity, TRPV4, TRPV1, Polyunsaturated fatty acid

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(09)00049-0

doi:10.1016/j.febslet.2009.01.027

FEBS Letters
Volume 583, Issue 4 , Pages 754-758, 18 February 2009

Article Tools

* Image Usage & Resolution