Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol

Marzesco, Anne-Marie; Wilsch-Bräuninger, Michaela; Dubreuil, Véronique; Janich, Peggy; Langenfeld, Katja; Thiele, Christoph; Huttner, Wieland B.; Corbeil, Denis

doi:10.1016/j.febslet.2009.01.048

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Volume 583, Issue 5 , Pages 897-902, 4 March 2009

Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol

Edited by Sandro Sonnino

Anne-Marie Marzesco
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Michaela Wilsch-Bräuninger
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Véronique Dubreuil
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
- Present address: UMR 8542, CNRS, Ecole Normale Supérieure, 46, rue d’Ulm, F-75005 Paris, France.
Peggy Janich
- Tissue Engineering Laboratories, BIOTEC and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany
Katja Langenfeld
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Christoph Thiele
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Wieland B. Huttner
- Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
- Corresponding authors. Fax: +49 (0) 351 463 40244 (D. Corbeil), +49 (0) 351 210 1600 (W.B. Huttner).
Denis Corbeil
- Tissue Engineering Laboratories, BIOTEC and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany
- Corresponding authors. Fax: +49 (0) 351 463 40244 (D. Corbeil), +49 (0) 351 210 1600 (W.B. Huttner).

Received 28 November 2008; received in revised form 24 January 2009; accepted 27 January 2009. published online 03 February 2009.

Abstract

We previously reported on the occurrence of prominin-1-carrying membrane vesicles that are released into body fluids from microvilli of epithelial cells. This release has been implicated in cell differentiation. Here we have characterized these vesicles released from the differentiated Caco-2 cells. We find that in these vesicles, prominin-1 directly interacts with membrane cholesterol and is associated with a membrane microdomain. The cholesterol depletion using methyl-β-cyclodextrin resulted in a marked increase in their release, and a dramatic change in the microvillar ultrastructure from a tubular shape to a “pearling” state, with multiple membrane constrictions, suggesting a role of membrane cholesterol in vesicle release from microvilli.

Keywords: Prominin, CD133, Cholesterol, Microvilli, Epithelial cell, Cancer