Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Taura, Daisuke; Noguchi, Michio; Sone, Masakatsu; Hosoda, Kiminori; Mori, Eisaku; Okada, Yohei; Takahashi, Kazutoshi; Homma, Koichiro; Oyamada, Naofumi; Inuzuka, Megumi; Sonoyama, Takuhiro; Ebihara, Ken; Tamura, Naohisa; Itoh, Hiroshi; Suemori, Hirofumi; Nakatsuji, Norio; Okano, Hideyuki; Yamanaka, Shinya; Nakao, Kazuwa

doi:10.1016/j.febslet.2009.02.031

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Volume 583, Issue 6 , Pages 1029-1033, 18 March 2009

Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Edited by Robert Barouki

Daisuke Taura
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
- These authors contributed equally to this work.
Michio Noguchi
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
- These authors contributed equally to this work.
Masakatsu Sone
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
- Corresponding authors. Fax: +81 75 771 9452.
Kiminori Hosoda
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
- Corresponding authors. Fax: +81 75 771 9452.
Eisaku Mori
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Yohei Okada
- Department of Physiology, Keio University, School of Medicine, Tokyo, Japan
Kazutoshi Takahashi
- Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- Center for iPS Cell Research and Application (CiRA), Institute for Integrated Cell-Material Sciences, Kyoto, Japan
Koichiro Homma
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
- Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan
Naofumi Oyamada
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Megumi Inuzuka
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Takuhiro Sonoyama
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Ken Ebihara
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Naohisa Tamura
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Hiroshi Itoh
- Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan
Hirofumi Suemori
- Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Norio Nakatsuji
- Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan
Hideyuki Okano
- Department of Physiology, Keio University, School of Medicine, Tokyo, Japan
Shinya Yamanaka
- Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- Center for iPS Cell Research and Application (CiRA), Institute for Integrated Cell-Material Sciences, Kyoto, Japan
Kazuwa Nakao
- Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan

Received 28 December 2008; received in revised form 10 February 2009; accepted 21 February 2009. published online 27 February 2009.

Abstract

Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12days of embryoid body formation and an additional 10days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.