FEBS Letters
Volume 583, Issue 6 , Pages 978-982, 18 March 2009

Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall

Edited by Ulf-Ingo Flügge

  • Jaap Timmers

      Affiliations

    • CRC-Wageningen, Wageningen University and Research Centre, 6703 HD Wageningen, The Netherlands
    • Wageningen UR Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands
    • Graduate School Experimental Plant Sciences, Wageningen University, The Netherlands
    • ,
  • Samantha Vernhettes

      Affiliations

    • Laboratoire de biologie Cellulaire, Institute Jean-Pierre Bourgin, INRA, Rte de Saint Cyr, Versailles, 78026 cedex, France
    • ,
  • Thierry Desprez

      Affiliations

    • Laboratoire de biologie Cellulaire, Institute Jean-Pierre Bourgin, INRA, Rte de Saint Cyr, Versailles, 78026 cedex, France
    • ,
  • Jean-Paul Vincken

      Affiliations

    • Wageningen UR Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands
    • Present address: Laboratory of Food Chemistry, Wageningen University, 6700 EV Wageningen, The Netherlands.
    • ,
  • Richard G.F. Visser

      Affiliations

    • Wageningen UR Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands
    • ,
  • Luisa M. Trindade

      Affiliations

    • Wageningen UR Plant Breeding, Wageningen University and Research Centre, 6708 PD Wageningen, The Netherlands
    • Corresponding Author InformationCorresponding author. Fax: +31 317 483457.

Received 5 December 2008; received in revised form 20 February 2009; accepted 24 February 2009. published online 02 March 2009.

Abstract 

It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins.

Structured summary

MINT-6951243: PIP2-1 (uniprotkb:P43286) physically interacts (MI:0218) with PIP2-1 (uniprotkb:P43286) by bimolecular fluorescence complementation (MI:0809)

MINT-6950816: CESA4 (uniprotkb:Q84JA6) physically interacts (MI:0218) with CESA4 (uniprotkb:Q84JA6) by membrane bound complementation assay (MI:0230)

MINT-6951056, MINT-6951071, MINT-6951088, MINT-6951103: CESA7 (uniprotkb:Q9SWW6) physically interacts (MI:0218) with CESA4 (uniprotkb:Q84JA6) by bimolecular fluorescence complementation (MI:0809)

MINT-6950949, MINT-6950990: CESA4 (uniprotkb:Q84JA6) physically interacts (MI:0218) with CESA8 (uniprotkb:Q8LPK5) by membrane bound complementation assay (MI:0230)

MINT-6950909, MINT-6951030: CESA4 (uniprotkb:Q8LPK5) physically interacts (MI:0218) with CESA7 (uniprotkb:Q9SWW6) by membrane bound complementation assay (MI:0230)

MINT-6951042: CESA4 (uniprotkb:Q84JA6) physically interacts (MI:0218) with CESA4 (uniprotkb:Q84JA6) by bimolecular fluorescence complementation (MI:0809)

MINT-6951004, MINT-6951016: CESA8 (uniprotkb:Q8LPK5) physically interacts (MI:0218) with CESA7 (uniprotkb:Q9SWW6) by membrane bound complementation assay (MI:0230)

MINT-6951217, MINT-6951230: CESA4 (uniprotkb:Q84JA6) physically interacts (MI:0218) with CESA8 (uniprotkb:Q8LPK5) by bimolecular fluorescence complementation (MI:0809)

MINT-6951120, MINT-6951140, MINT-6951156, MINT-6951170, MINT-6951185: CESA8 (uniprotkb:Q8LPK5) physically interacts (MI:0218) with CESA7 (uniprotkb:Q9SWW6) by bimolecular fluorescence complementation (MI:0809)

MINT-6951199: CESA8 (uniprotkb:Q8LPK5) physically interacts (MI:0218) with CESA8 (uniprotkb:Q8LPK5) by bimolecular fluorescence complementation (MI:0809)

Abbreviations: MbYTH, membrane-based yeast two hybrid, CESA, cellulose synthase, BiFC, bimolecular fluorescence complementation, TMD, transmembrane domain, TF, transcription factor, YFP, yellow fluorescent protein

Keywords: Secondary cell wall, Protein–protein interaction, CESA, MbYTH, BiFC

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PII: S0014-5793(09)00149-5

doi:10.1016/j.febslet.2009.02.035

FEBS Letters
Volume 583, Issue 6 , Pages 978-982, 18 March 2009

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