Association of the eukaryotic V1VO ATPase subunits a with d and d with A

Thaker, Youg R.; Hunke, Cornelia; Yau, Yin H.; Shochat, Susana Geifman; Li, Ying; Grüber, Gerhard

doi:10.1016/j.febslet.2009.03.013

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Volume 583, Issue 7 , Pages 1090-1095, 2 April 2009

Association of the eukaryotic V₁V_O ATPase subunits a with d and d with A

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Republic of Singapore

Received 23 January 2009; received in revised form 4 March 2009; accepted 5 March 2009. published online 16 March 2009.

Edited by Peter Brzezinski

Abstract

Owing to the complex nature of V₁V_O ATPases, identification of neighboring subunits is essential for mechanistic understanding of this enzyme. Here, we describe the links between the V₁ headpiece and the V_O-domain of the yeast V₁V_O ATPase via subunit A and d as well as the V_O subunits a and d using surface plasmon resonance and fluorescence correlation spectroscopy. Binding constants of about 60 and 200nM have been determined for the a–d and d–A assembly, respectively. The data are discussed in light of subunit a and d forming a peripheral stalk, connecting the catalytic A₃B₃ hexamer with V_O.