Assembly of a chimeric respiratory chain from bovine heart submitochondrial particles and cytochrome bd terminal oxidase of Escherichia coli

Gavrikova, Eleonora V.; Grivennikova, Vera G.; Borisov, Vitaliy B.; Cecchini, Gary; Vinogradov, Andrei D.

doi:10.1016/j.febslet.2009.03.022

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Volume 583, Issue 8 , Pages 1287-1291, 17 April 2009

Assembly of a chimeric respiratory chain from bovine heart submitochondrial particles and cytochrome bd terminal oxidase of Escherichia coli

Edited by Peter Brzezinski

Eleonora V. Gavrikova
- Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation
Vera G. Grivennikova
- Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation
Vitaliy B. Borisov
- Department of Molecular Energetics of Microorganisms, Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119991, Russian Federation
Gary Cecchini
- Molecular Biology Division, VA Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA
- Department of Biochemistry & Biophysics, University of California, San Francisco, CA 94158, USA
Andrei D. Vinogradov
- Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation
- Mitoengineering Center of Moscow State University, Moscow 119991, Russian Federation
- Corresponding author. Address: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation. Fax: +7 495 939 1376.

Received 27 January 2009; received in revised form 4 March 2009; accepted 12 March 2009. published online 19 March 2009.

Abstract

Cytochrome bd is a terminal quinol oxidase in Escherichia coli. Mitochondrial respiration is inhibited at cytochrome bc₁ (complex III) by myxothiazol. Mixing purified cytochrome bd oxidase with myxothiazol-inhibited bovine heart submitochondrial particles (SMP) restores up to 50% of the original rotenone-sensitive NADH oxidase and succinate oxidase activities in the absence of exogenous ubiquinone analogs. Complex III bypassed respiration and is saturated at amounts of added cytochrome bd similar to that of other natural respiratory components in SMP. The cytochrome bd tightly binds to the mitochondrial membrane and operates as an intrinsic component of the chimeric respiratory chain.

Abbreviation: SMP, submitochondrial particles

Keywords: Complex I, Complex II, Cytochrome bd quinol oxidase, Ubiquinone oxidoreduction, Respiratory chain, Mitochondria