The effect of 2-deoxy-d-glucose on Werner syndrome RecQ helicase gene

Abstract 

Caloric restriction (CR) is known to effectively elongate mammalian life-spans. The compound 2-deoxy-d-glucose (2DG), which is often used as an inhibitor of glucose utilization, is a mimetic agent of CR. In this study, we examined the changes of telomerase and Werner’s syndrome RecQ (WRN) helicase after treatment with 2DG, because of the involvement of recQ helicase in the regulation of telomeres. Interestingly, 2DG treatment increased the expression of WRN protein in accordance with induction of its promoter activity and gene expression. Furthermore, the activation of telomerase was observed after 2DG treatment, whereas it resulted in the reduction of cell proliferation. These results suggest that 2DG could up-regulate telomere maintenance factors accompanied with suppression of proliferation.

Abbreviations: BS, Bloom’s syndrome, CR, caloric restriction, 2DG, 2-deoxy-d-glucose, Luc, luciferase, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, RT-PCR, reverse transcriptase polymerase chain reaction, TERT, telomerase reverse transcriptase, TRAP, telomerase amplification protocol, WS, Werner’s syndrome

Keywords: BLM, 2DG, HELB, p21, RecQ helicase, Sp1, Telomerase, TERT, WRN