FEBS Letters
Volume 583, Issue 9 , Pages 1457-1462, 6 May 2009

A novel family of hemicellulolytic α-glucuronidase

Edited by Judit Ovádi

  • Olena Ryabova

      Affiliations

    • Institute of Chemistry, Slovak Academy of Sciences, Department of Carbohydrate Enzymology, Dúbravská cesta 9, 845 38 Bratislava 45, Slovakia
    • ,
  • Mária Vršanská

      Affiliations

    • Institute of Chemistry, Slovak Academy of Sciences, Department of Carbohydrate Enzymology, Dúbravská cesta 9, 845 38 Bratislava 45, Slovakia
    • ,
  • Satoshi Kaneko

      Affiliations

    • National Food Research Institute, 2-1-12 Kannondai, Tsukuba 305-8642, Ibaraki, Japan
    • ,
  • Willem H. van Zyl

      Affiliations

    • Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa
    • ,
  • Peter Biely

      Affiliations

    • Institute of Chemistry, Slovak Academy of Sciences, Department of Carbohydrate Enzymology, Dúbravská cesta 9, 845 38 Bratislava 45, Slovakia
    • Corresponding Author InformationCorresponding author. Fax: +421 2 59410 222.

Received 9 February 2009; received in revised form 11 March 2009; accepted 25 March 2009. published online 01 April 2009.

Abstract 

Investigation of the xylanolytic enzyme system of the xylose-fermenting yeast Pichia stipitis resulted in the discovery of an extracellular α-glucuronidase efficiently debranching hardwood glucuronoxylan. This activity is not exhibited by more extensively investigated α-glucuronidases of glycoside hydrolase (GH) family 67, operating on substrates in which the uronic acid is linked to the non-reducing xylopyranosyl residues of main chain fragments. The N-terminus of the purified enzyme corresponded exactly to the P. stipitis gene ABN67901 coding for a protein of unknown function. BLAST search revealed the presence of similar genes in genomes of other microorganisms. These results lead to the emergence of a new family of α-glucuronidases.

Keywords: α-Glucuronidase, Glycoside hydrolase, Xylanolytic enzyme, Pichia stipitis

Abbreviations: MeGlcA, 4-O-methyl-d-glucuronic acid, GH, glycoside hydrolase

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PII: S0014-5793(09)00249-X

doi:10.1016/j.febslet.2009.03.057

FEBS Letters
Volume 583, Issue 9 , Pages 1457-1462, 6 May 2009

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