Current trends in high throughput proteomics in cyanobacteria

Abstract 

Advancements in genome sequencing and high throughput proteomics of cyanobacterial strains led to 13 published reports, from a small number of laboratories. These successful studies focused on Synechocystis, Nostoc and Anabaena strains, prochlorococcus, and halotolerant Euhalothece. The implications of emerging quantitative aspects developed and applied in these large-scale studies are assessed in the wake of advanced cyanobacterial research. Furthermore, contributions from traditional and early high throughput analysis of cyanobacterial proteomics are compared and summarised. Finally, opinions are provided to link both the trends and the future challenges. This review aims to push the synergy between proteomics and cyanobacterial research to improve both the technical and biological significance.

Abbreviations: HTT, high throughput techniques, MS, mass spectrometry or tandem mass spectrometry, MS/MS, fragment peptide scan, LC, liquid chromatography, 2DGE, two-dimensional gel electrophoresis, 1DGE, one-dimensional gel electrophoresis, MALDI, matrix assisted laser desorption ionisation, MALDI-TOF/TOF, MALDI interface-tandem time-of-flight analyser, Qq-TOF, electrospray quadrupole time-of-flight analyser, Q-IT, electrospray quadrupole ion trap analyser, LC-MALDI, liquid chromatography MALDI spotting interface, m/z, mass over charge ratio, ICAT, isotope coded affinity tags, iTRAQ, isobaric tags for relative and absolute quantification

Keywords: Shotgun proteomics, High throughput proteomics, Cyanobacteria proteomics, Cyanobacteria, Post-genomic study