The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: Molecular and physiological characterization of PEPC kinase mutants

Meimoun, Patrice; Gousset-Dupont, Aurélie; Lebouteiller, Bénédicte; Ambard-Bretteville, Françoise; Besin, Evelyne; Lelarge, Caroline; Mauve, Caroline; Hodges, Michael; Vidal, Jean

doi:10.1016/j.febslet.2009.04.030

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Volume 583, Issue 10 , Pages 1649-1652, 19 May 2009

The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: Molecular and physiological characterization of PEPC kinase mutants

Edited by Michael R. Sussman

Patrice Meimoun
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
- Authors having equally contributed to this work.
Aurélie Gousset-Dupont
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
- Authors having equally contributed to this work.
Bénédicte Lebouteiller
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Françoise Ambard-Bretteville
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Evelyne Besin
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Caroline Lelarge
- Plate-forme Métabolisme-Métabolome, Institut Fédératif de Recherche 87, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Caroline Mauve
- Plate-forme Métabolisme-Métabolome, Institut Fédératif de Recherche 87, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Michael Hodges
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
Jean Vidal
- Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay Cedex, France
- Corresponding author. Fax: +33 1 69153423.

Received 28 January 2009; received in revised form 9 April 2009; accepted 20 April 2009. published online 27 April 2009.

Abstract

Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard in growth under our greenhouse conditions. A mass-spectrometry-based analysis revealed significant changes in metabolite profiles. However, the anaplerotic pathway initiated by PEPC was only moderately altered. These data establish the PPCk1 gene product as responsible for leaf PEPC phosphorylation in planta and show that the absence of PEPC phosphorylation has pleiotropic consequences on plant metabolism.

Abbreviations: PEPC, phosphoenolpyruvate carboxylase, PEPCk, PEPC kinase, CAM, crassulacean acid metabolism, WT, wild-type, P-PEPC, phosphorylated-PEPC, OAA, oxaloacetate