Regulation of voltage-gated K+ channels by glucose metabolism in pancreatic β-cells

Yoshida, Masashi; Dezaki, Katsuya; Yamato, Shiho; Aoki, Atsushi; Sugawara, Hitoshi; Toyoshima, Hideo; Ishikawa, San-e; Kawakami, Masanobu; Nakata, Masanori; Yada, Toshihiko; Kakei, Masafumi

doi:10.1016/j.febslet.2009.05.050

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Volume 583, Issue 13 , Pages 2225-2230, 7 July 2009

Regulation of voltage-gated K⁺ channels by glucose metabolism in pancreatic β-cells

Edited by Maurice Montal

Masashi Yoshida
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Katsuya Dezaki
- Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Tochigi 329-0423, Japan
Shiho Yamato
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Atsushi Aoki
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Hitoshi Sugawara
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Hideo Toyoshima
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
San-e Ishikawa
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Masanobu Kawakami
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
Masanori Nakata
- Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Tochigi 329-0423, Japan
Toshihiko Yada
- Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Tochigi 329-0423, Japan
Masafumi Kakei
- First Department of Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, Omiya 1-847, Saitama 337-8503, Japan
- Corresponding author. Fax: +81 48 648 5188.

Received 17 April 2009; received in revised form 28 May 2009; accepted 29 May 2009. published online 03 June 2009.

Abstract

Regulation of delayed rectifier-type K⁺ channels (Kv-channels) by glucose was studied in rat pancreatic β-cells. The Kv-channel current was increased in amplitudes by increasing glucose concentration from 2.8 to 16.6mM, while it was decreased by 2.8mM glucose in a reversible manner (down-regulation) in both perforated and conventional whole-cell modes. The current was decreased by FCCP, intrapipette 0mM ATP or AMPPNP. Glyceraldehyde, pyruvic acid, 2-ketoisocaproic acid, and 10mM MgATP prevented the down-regulation induced by 2.8mM or less glucose. The residual current after treatment with Kv2.1-specific blocker, guangxitoxin-1E, was unchanged by lowering or increasing glucose concentration. We conclude that glucose metabolism regulates Kv2.1 channels in rats β-cells via altering MgATP levels.