FEBS Letters
Volume 583, Issue 13 , Pages 2165-2170, 7 July 2009

TOE1 interacts with p53 to modulate its transactivation potential

Edited by Varda Rotter

  • Sabina Sperandio

      Affiliations

    • Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada
    • ,
  • Saverio Tardito

      Affiliations

    • Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada
    • ,
  • Aleksandra Surzycki

      Affiliations

    • Indiana University, Bloomington, IN, USA
    • ,
  • Martin Latterich

      Affiliations

    • University of Montreal, Faculty of Pharmacy, Montreal, QC Canada
    • Present address: Biomatrica Inc., 5627 Oberlin Drive, Suite 120, San Diego, CA 92121, USA.
    • ,
  • Ian de Belle

      Affiliations

    • Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada
    • Corresponding Author InformationCorresponding author.

Received 2 April 2009; received in revised form 11 May 2009; accepted 2 June 2009. published online 08 June 2009.

Abstract 

The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetramerization domain of p53. We have further characterized this interaction through determination of binding kinetics using nanoporous optical interferometry and demonstrated that this interaction is capable of enhancing the transcriptional activity of p53-dependent gene targets. These results suggest a mechanistic role for TOE1 as a co-regulator of p53.

Structured summary

MINT-7144895: P53 (uniprotkb:P04637) physically interacts (MI:0914) with TOE1 (uniprotkb:Q96GM8) by pull down (MI:0096)

MINT-7144912: TOE1 (uniprotkb:Q96GM8) binds (MI:0407) to P53 (uniprotkb:P04637) by biophysical (MI:0013)

MINT-7144884: P53 (uniprotkb:P04637) physically interacts (MI:0914) with TOE1 (uniprotkb:Q96GM8) by anti bait coimmunoprecipitation (MI:0006)

Keyword: Protein interaction

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PII: S0014-5793(09)00445-1

doi:10.1016/j.febslet.2009.06.004

FEBS Letters
Volume 583, Issue 13 , Pages 2165-2170, 7 July 2009

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