Cyclin Y, a novel membrane-associated cyclin, interacts with PFTK1

Jiang, Mei; Gao, Yankun; Yang, Tao; Zhu, Xueliang; Chen, Jiangye

doi:10.1016/j.febslet.2009.06.010

« Previous Next »FEBS Letters
Volume 583, Issue 13 , Pages 2171-2178, 7 July 2009

Cyclin Y, a novel membrane-associated cyclin, interacts with PFTK1

Edited by Angel Nebreda

Mei Jiang
- State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Yankun Gao
- State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Tao Yang
- State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Xueliang Zhu
- Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Jiangye Chen
- State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
- Corresponding author. Fax: +86 21 54921011.

Received 29 April 2009; received in revised form 1 June 2009; accepted 4 June 2009. published online 12 June 2009.

Abstract

A novel cyclin, CCNY, was identified as a PFTK1 interacting protein in a yeast two-hybrid screen. The cyclin box in CCNY and the PFTAIRE motif in PFTK1 are both required for the interaction which was confirmed by in vivo and in vitro assays. Two transcripts (4 and 2kb), of CCNY were detected by Northern blot analysis and CCNY was enriched at the plasma membrane due to an N-terminal myristoylation signal. We propose that binding of CCNY to PFTK1 enhances PFTK1 kinase activity and changes its intracellular location.

Structured summary

MINT-7147585, MINT-7147598, MINT-7147614, MINT-7147628, MINT-7147647, MINT-7147665, MINT-7147680: pftk1 (uniprotkb:O94921) physically interacts (MI:0915) with CCNY (uniprotkb:Q8ND76) by two hybrid (MI:0018)

MINT-7147725, MINT-7147743: pftk1 (uniprotkb:O94921) physically interacts (MI:0914) with CCNY (uniprotkb:Q8ND76) by anti tag coimmunoprecipitation (MI:0007)

MINT-7147758: pftk1 (uniprotkb:O35495) physically interacts (MI:0914) with CCNY (uniprotkb:Q8BGU5) by anti bait coimmunoprecipitation (MI:0006)

MINT-7147695, MINT-7147713: pftk1 (uniprotkb:O94921) and CCNY (uniprotkb:Q8ND76) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

Keywords: CCNY, PFTK1, Membrane localization, Interaction, Regulatory partner, Kinase activity