FEBS Letters
Volume 583, Issue 13 , Pages 2213-2217, 7 July 2009

Dexamethasone regulates expression of BRUCE/Apollon and the proliferation of neural progenitor cells

Edited by Ned Mantei

  • Maria Sippel

      Affiliations

    • Minerva Medical Research Institute, Biomedicum, Tukholmankatu 8, Helsinki, Finland
    • ,
  • Raili Rajala

      Affiliations

    • Minerva Medical Research Institute, Biomedicum, Tukholmankatu 8, Helsinki, Finland
    • ,
  • Laura Korhonen

      Affiliations

    • Minerva Medical Research Institute, Biomedicum, Tukholmankatu 8, Helsinki, Finland
    • ,
  • Beat Bornhauser

      Affiliations

    • Department of Neuroscience, Neurobiology, BMC, Uppsala University, Uppsala, Sweden
    • Department of Oncology, Children’s Hospital, University of Zurich, Zurich, Switzerland
    • ,
  • Anna-Leena Sokka

      Affiliations

    • Minerva Medical Research Institute, Biomedicum, Tukholmankatu 8, Helsinki, Finland
    • ,
  • Mikihiko Naito

      Affiliations

    • Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
    • ,
  • Dan Lindholm

      Affiliations

    • Minerva Medical Research Institute, Biomedicum, Tukholmankatu 8, Helsinki, Finland
    • Department of Neuroscience, Neurobiology, BMC, Uppsala University, Uppsala, Sweden
    • Corresponding Author InformationCorresponding author. Address: Minerva Medical Research Institute, Biomedicum-2 Helsinki, Tukholmankatu 8, FIN-00290 Helsinki, Finland. Fax: +358 9 19125701.

Received 27 May 2009; received in revised form 7 June 2009; accepted 8 June 2009. published online 15 June 2009.

Abstract 

Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing RNA reduced the number of proliferating NPCs, whilst overexpression of BRUCE counteracted the effect of Dex. Dex also elevated the deubiquitinating enzyme, Usp8/Ubpy, which via Nrdp1 decreases BRUCE. The results show that BRUCE is a target for GHs in the NPCs, and that BRUCE controls cell division of NPCs and possibly of other stem cells.

Structured summary

MINT-7148564: Nrdp1 (uniprotkb:Q8BH75) physically interacts (MI:0914) with BRUCE (uniprotkb:O88738) by anti bait co-immunoprecipitation (MI:0006)

MINT-7148555: Nrdp1 (uniprotkb:Q8BH75) physically interacts (MI:0914) with Usp8 (uniprotkb:Q80U87) by anti bait co-immunoprecipitation (MI:0006)

Abbreviations: AS, antisense, BIR, baculovirus inhibitory repeat, BRUCE/Apollon, baculoviral inhibitor of apoptosis repeat-containing 6 gene, Dex, dexamethasone, E, embryonic day, EGF, epidermal growth factor, GHs, glucocorticoid hormones, IAP, inhibitor of apoptosis protein, NPCs, neural progenitor cells, siRNA, silencing RNA

Keywords: Neural progenitor cell, Hormone, Cell proliferation, BRUCE, Usp8, Silencing RNA

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PII: S0014-5793(09)00465-7

doi:10.1016/j.febslet.2009.06.018

FEBS Letters
Volume 583, Issue 13 , Pages 2213-2217, 7 July 2009

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