FEBS Letters
Volume 583, Issue 14 , Pages 2333-2338, 21 July 2009

Evidence for a degradosome-like complex in the mitochondria of Trypanosoma brucei

Edited by Michael Ibba

  • Jonelle L. Mattiacio

      Affiliations

    • Present address: University of Rochester, Department of Microbiology and Immunology, 601 Elmwood Ave, Box 672, KMRB, Room 3-9804, Rochester, NY 14642, USA.
    • ,
  • Laurie K. Read

      Affiliations

    • Corresponding Author InformationCorresponding author. Fax: +1 716 829 2158.

Depatment of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, SUNY Buffalo School of Medicine, Buffalo, NY 14214, USA

Received 29 May 2009; accepted 11 June 2009. published online 18 June 2009.

Abstract 

Mitochondrial RNA turnover in yeast involves the degradosome, composed of DSS-1 exoribonuclease and SUV3 RNA helicase. Here, we describe a degradosome-like complex, containing SUV3 and DSS-1 homologues, in the early branching protozoan, Trypanosoma brucei. TbSUV3 is mitochondrially localized and co-sediments with TbDSS-1 on glycerol gradients. Co-immunoprecipitation demonstrates that TbSUV3 and TbDSS-1 associate in a stable complex, which differs from the yeast degradosome in that it is not stably associated with mitochondrial ribosomes. This is the first report of a mitochondrial degradosome-like complex outside of yeast. Our data indicate an early evolutionary origin for the mitochondrial SUV3/DSS-1 containing complex.

Structured summary

MINT-7187980: SUV3 (genbank_protein_gi:XP_844349) and DSS1 (uniprotkb:Q38EM3) colocalize (MI:0403) by cosedimentation (MI:0027)

MINT-7188074: SUV3 (genbank_protein_gi:XP_844349) physically interacts (MI:0914) with DSS1 (uniprotkb:Q38EM3) by anti tag co-immunoprecipitation (MI:0007)

Keywords: RNA turnover, RNA processing, Trypanosome, RNA helicase, Exoribonuclease

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PII: S0014-5793(09)00472-4

doi:10.1016/j.febslet.2009.06.024

FEBS Letters
Volume 583, Issue 14 , Pages 2333-2338, 21 July 2009

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