MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells

Arnaud, Camille; Sebbagh, Michaël; Nola, Sébastien; Audebert, Stéphane; Bidaut, Ghislain; Hermant, Aurélie; Gayet, Odile; Dusetti, Nelson J.; Ollendorff, Vincent; Santoni, Marie-Josée; Borg, Jean-Paul; Lécine, Patrick

doi:10.1016/j.febslet.2009.06.034

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Volume 583, Issue 14 , Pages 2326-2332, 21 July 2009

MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells

Edited by Beat Imhof

Camille Arnaud
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
- These authors contributed equally to this work.
- Present address: Consulate General of France in Los Angeles, 10390 Santa Monica Blvd, Suite 410, Los Angeles, CA 90025, USA.
Michaël Sebbagh
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
- These authors contributed equally to this work.
Sébastien Nola
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
- Present address: Molecular Medicine, National Heart and Lung Institute Faculty of Medicine, Imperial College London, London SW7 2AZ, UK.
Stéphane Audebert
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
Ghislain Bidaut
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
Aurélie Hermant
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
- Present address: Merck Serono International SA, 9 Chemin des Mines, Case Postale 54, 1211 Genève 20, Switzerland.
Odile Gayet
- INSERM U624, Stress Cellulaire, Marseille, France
- Aix-Marseille Université, Campus de Luminy, Marseille, France
Nelson J. Dusetti
- INSERM U624, Stress Cellulaire, Marseille, France
- Aix-Marseille Université, Campus de Luminy, Marseille, France
Vincent Ollendorff
- INRA, UMR866 Différentiation Cellulaire et Croissance, Montpellier, France
Marie-Josée Santoni
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
Jean-Paul Borg
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
Patrick Lécine
- INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France
- Institut Paoli-Calmettes, Marseille F-13009, France
- Univ. Méditerranée, Marseille F-13007, France
- Corresponding author. Present address: Baylor Institute for Immunology Research, INSERM UMR899, 3434 Live Oak Street, Dallas, TX 75024, USA. Fax: +1 214 820 4813.

Received 25 March 2009; received in revised form 14 June 2009; accepted 17 June 2009. published online 23 June 2009.

Abstract

To further characterize the molecular events supporting the tumor suppressor activity of Scrib in mammals, we aim to identify new binding partners. We isolated MCC, a recently identified binding partner for β-catenin, as a new interacting protein for Scrib. MCC interacts with both Scrib and the NHERF1/NHERF2/Ezrin complex in a PDZ-dependent manner. In T47D cells, MCC and Scrib proteins colocalize at the cell membrane and reduced expression of MCC results in impaired cell migration. By contrast to Scrib, MCC inhibits cell directed migration independently of Rac1, Cdc42 and PAK activation. Altogether, these results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, β-catenin and NHERF1/2.

Structured summary

MINT-7211022: SCRIB (uniprotkb:Q14160) and MCC (uniprotkb:P23508) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

MINT-7210609: SCRIB (uniprotkb:Q14160) physically interacts (MI:0915) with MCC (uniprotkb:P23508) by two hybrid (MI:0018)

MINT-7210759, MINT-7210792: SCRIB (uniprotkb:Q14160) physically interacts (MI:0914) with PIX beta (uniprotkb:Q14155) by pull down (MI:0096)

MINT-7210883, MINT-7210820: SCRIB (uniprotkb:Q14160) physically interacts (MI:0914) with MCC (uniprotkb:P23508) by anti bait coimmunoprecipitation (MI:0006)

MINT-7210634, MINT-7210690, MINT-7210731: SCRIB (uniprotkb:Q14160) physically interacts (MI:0914) with MCC (uniprotkb:P23508) by pull down (MI:0096)

MINT-7211267: E6 (uniprotkb:P06463) physically interacts (MI:0915) with SCRIB (uniprotkb:Q14160), SNX27 (uniprotkb:Q96L92), UTRN (uniprotkb:P46939), CASK (uniprotkb:O14936), DMD (uniprotkb:P11532) and Dlg (uniprotkb:Q12959) by pull down (MI:0096)

MINT-7211237: MCC (uniprotkb:P23508) physically interacts (MI:0915) with SCRIB (uniprotkb:Q14160), EZR (uniprotkb:P15311), SNX27 (uniprotkb:Q96L92), NHERF1 (uniprotkb:O14745) and NHERF2 (uniprotkb:Q15599) by pull down (MI:0096)