Syndecan-4 promotes the retention of phosphatidylinositol 4,5-bisphosphate in the plasma membrane

Kwon, Soojin; Son, Hyowon; Choi, Youngsil; Lee, Jung-hyun; Choi, Sojoong; Lim, Yangmi; Han, Inn-Oc; Oh, Eok-Soo

doi:10.1016/j.febslet.2009.06.039

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Volume 583, Issue 14 , Pages 2395-2400, 21 July 2009

Syndecan-4 promotes the retention of phosphatidylinositol 4,5-bisphosphate in the plasma membrane

Edited by Sandro Sonnino

Soojin Kwon
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
Hyowon Son
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
Youngsil Choi
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
Jung-hyun Lee
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
Sojoong Choi
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
Yangmi Lim
- Mogam Biotechnology Research Institute, Yongin, 446-799, Republic of Korea
Inn-Oc Han
- Department of Physiology and Biophysics, Inha University, Incheon 402-751, Republic of Korea
Eok-Soo Oh
- Department of Life Sciences, Division of Life and Pharmaceutical Sciences, The Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750, Republic of Korea
- Corresponding author. Fax: +82 2 3277 3760.

Received 20 March 2009; received in revised form 22 June 2009; accepted 22 June 2009. published online 26 June 2009.

Abstract

Although phosphatidylinositol 4,5-bisphosphate (PIP₂) regulates syndecan-4 function, the potential influence of syndecan-4 on PIP₂ remains unknown. GFP containing PIP₂-binding-PH domain of phospholipase Cδ (GFP-PHδ) was used to monitor PIP₂. Syndecan-4 overexpression in COS-7 cells enhanced membrane translocation of GFP-PHδ, while the opposite was observed when syndecan-4 was knocked-down. PIP₂ levels were higher in total phospholipids extracted from rat embryo fibroblasts expressing syndecan-4. Syndecan-4-induced membrane targeting of GFP-PHδ was further enhanced by phosphoinositide-3-kinase inhibitor, but not by phospholipase C (PLC) inhibitor. Besides, both ionomycin and epidermal growth factor caused dissociation of GFP-PHδ from plasma membrane, an effect that was significantly delayed by syndecan-4 over-expression. Collectively, these data suggest that syndecan-4 promotes plasma membrane retention of PIP₂ by negatively regulating PLC-dependent PIP₂ degradation.

Keywords: Syndecan, Adhesion, Phosphatidylinositol 4,5-bisphosphate, Signal transduction