FEBS Letters
Volume 583, Issue 16 , Pages 2654-2662, 20 August 2009

The GroEL/GroES cis cavity as a passive anti-aggregation device

Edited by Per Hannarström

  • Arthur L. Horwich

      Affiliations

    • Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States
    • Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States
    • Corresponding Author InformationCorresponding author. Address: Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States. Fax: +1 203 737 1761.
    • ,
  • Adrian C. Apetri

      Affiliations

    • Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States
    • Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States
    • ,
  • Wayne A. Fenton

      Affiliations

    • Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States

Received 10 March 2009; received in revised form 27 June 2009; accepted 30 June 2009. published online 03 July 2009.

Abstract 

The GroEL/GroES chaperonin folding chamber is an encapsulated space of ∼65Å diameter with a hydrophilic wall, inside of which many cellular proteins reach the native state. The question of whether the cavity wall actively directs folding reactions or is playing a passive role has been open. We review past and recent observations and conclude that the chamber functions as a passive “Anfinsen cage” that prevents folding monomers from multimolecular aggregation.

Abbreviations: DM-MBP, double mutant of maltose-binding protein, GSH/GSSG, reduced and oxidized glutathione, respectively, TG, trypsinogen, BSA, bovine serum albumin, NMR, nuclear magnetic resonance, DHFR, dihydrofolate reductase, GFP, green fluorescent protein, R. rubrum, Rhodospirillum rubrum

Keywords: GroEL, Chaperonin, Aggregation, cis Cavity

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PII: S0014-5793(09)00510-9

doi:10.1016/j.febslet.2009.06.049

FEBS Letters
Volume 583, Issue 16 , Pages 2654-2662, 20 August 2009

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