| | The GroEL/GroES cis cavity as a passive anti-aggregation deviceEdited by Per Hannarström Received 10 March 2009; received in revised form 27 June 2009; accepted 30 June 2009. published online 03 July 2009. Abstract The GroEL/GroES chaperonin folding chamber is an encapsulated space of ∼65 Å diameter with a hydrophilic wall, inside of which many cellular proteins reach the native state. The question of whether the cavity wall actively directs folding reactions or is playing a passive role has been open. We review past and recent observations and conclude that the chamber functions as a passive “Anfinsen cage” that prevents folding monomers from multimolecular aggregation. Abbreviations: DM-MBP, double mutant of maltose-binding protein, GSH/GSSG, reduced and oxidized glutathione, respectively, TG, trypsinogen, BSA, bovine serum albumin, NMR, nuclear magnetic resonance, DHFR, dihydrofolate reductase, GFP, green fluorescent protein, R. rubrum, Rhodospirillum rubrum a Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States b Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States  Corresponding author. Address: Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Ave., New Haven, CT 06510, United States. Fax: +1 203 737 1761.
PII: S0014-5793(09)00510-9 doi:10.1016/j.febslet.2009.06.049 © 2009 Federation of European Biochemical Societies | |
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ABSTRACT
