Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

Nakamura, Nobuhisa; Naruse, Keiko; Matsuki, Takashi; Hamada, Yoji; Nakashima, Eitaro; Kamiya, Hideki; Matsubara, Tatsuaki; Enomoto, Atsushi; Takahashi, Masahide; Oiso, Yutaka; Nakamura, Jiro

doi:10.1016/j.febslet.2009.07.011

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Volume 583, Issue 15 , Pages 2457-2463, 6 August 2009

Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

Edited by Lukas Huber

Nobuhisa Nakamura
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
Keiko Naruse
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
- Corresponding author. Address: Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan. Fax: +81 52 744 2206.
Takashi Matsuki
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
Yoji Hamada
- Department of Metabolic Diseases, Nagoya University School of Medicine, Nagoya, Japan
Eitaro Nakashima
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
Hideki Kamiya
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
Tatsuaki Matsubara
- Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Atsushi Enomoto
- Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Japan
Masahide Takahashi
- Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Japan
Yutaka Oiso
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan
Jiro Nakamura
- Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan

Received 15 April 2009; received in revised form 7 July 2009; accepted 9 July 2009. published online 13 July 2009.

Abstract

Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1.