FEBS Letters
Volume 583, Issue 17 , Pages 2715-2719, 3 September 2009

Identification of MAFbx as a myogenin-engaged F-box protein in SCF ubiquitin ligase

Edited by Noboru Mizushima

  • Misako Jogo

      Affiliations

    • These authors contribute equally in this study.
    • ,
  • Seiji Shiraishi

      Affiliations

    • These authors contribute equally in this study.
    • ,
  • Taka-aki Tamura

      Affiliations

    • Corresponding Author InformationCorresponding author. Present address: Exploratory and Applied Pharmaceutical Research Department, Ajinomoto Co. Inc., Kawasaki 210-8681, Japan. Fax: +81 43 290 2824.

Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba 263-8522, Japan

Received 8 June 2009; received in revised form 1 July 2009; accepted 14 July 2009. published online 22 July 2009.

Abstract 

Myogenesis is conducted by transcription factors including MyoD and myogenin. Myogenin is known to be polyubiquitinated by SCF (Skp1/Cullin 1/F-box protein) followed by proteasomal degradation, though the participating F-box protein is remaining unidentified. In this study, we found that myogenin in differentiated myoblasts is destabilized by muscle atrophy-inducing dexamethasone and that MAFbx (muscle atrophy F-box protein) is increased in atrophying myotubes. MAFbx overexpression resulted in MG132-sensitive reduction of myogenin. Myogenin had a MAFbx-recognition motif and interacted with MAFbx. MAFbx activated polyubiquitination of myogenin. The results of this study suggest that MAFbx functions as an F-box protein for ubiquitination of myogenin.

Structured summary

MINT-7222713: Myogenin (uniprotkb:P12979) physically interacts (MI:0914) with MAFbx (uniprotkb:Q9CPU7) by anti tag coimmunoprecipitation (MI:0007)

MINT-7222741: Myogenin (uniprotkb:P12979) physically interacts (MI:0914) with MAFbx (uniprotkb:Q9CPU7) by anti bait coimmunoprecipitation (MI:0006)

MINT-7222726: Myogenin (uniprotkb:P12979) and MAFbx (uniprotkb:Q9CPU7) colocalize (MI:0403) by fluorescence microscopy (MI:0416)

MINT-7222760: Myogenin (uniprotkb:P12979) physically interacts (MI:0914) with Ubiquitin (uniprotkb:P62991) by anti bait coimmunoprecipitation (MI:0006)

Abbreviations: UPS, ubiquitin proteasome system, Ub, ubiquitin, MAFbx, muscle atrophy F-box protein, SCF, Skp1/Cullin 1/F-box protein, MuRF1, muscle RING-finger protein-1, Dex, dexamethasone, IGF-1, insulin-like growth factor-1, HA, hemagglutinin, siRNA, small interference RNA, GM, growth medium, DM, differentiation medium, PAGE, polyacrylamide gel electrophoresis, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, TBP, TATA-binding protein

Keywords: MAFbx, Atrogin-1, Ubiquitination, Myogenin, Myogenesis, SCF

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(09)00559-6

doi:10.1016/j.febslet.2009.07.033

FEBS Letters
Volume 583, Issue 17 , Pages 2715-2719, 3 September 2009

Article Tools

* Image Usage & Resolution