A cold-induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro

Xie, Guosheng; Kato, Hideki; Sasaki, Kentaro; Imai, Ryozo

doi:10.1016/j.febslet.2009.07.057

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Volume 583, Issue 17 , Pages 2734-2738, 3 September 2009

A cold-induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro

Edited by Julian Schroeder

Guosheng Xie
- Crop Cold Tolerance Team, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo 062-8555, Japan
- National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
- These authors contributed equally to this work.
Hideki Kato
- Crop Cold Tolerance Team, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo 062-8555, Japan
- These authors contributed equally to this work.
Kentaro Sasaki
- Crop Cold Tolerance Team, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo 062-8555, Japan
Ryozo Imai
- Crop Cold Tolerance Team, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, Sapporo 062-8555, Japan
- Corresponding author. Fax: +81 11 857 9382.

Received 28 May 2009; received in revised form 20 July 2009; accepted 29 July 2009. published online 06 August 2009.

Abstract

Cytosolic thioredoxins are small conserved proteins that are involved in cellular redox regulation. Here, we report that a major and cold-induced thioredoxin h of rice, OsTrx23, has an inhibitory activity on stress-activated mitogen-activated protein kinases (MAPKs), OsMPK3 and OsMPK6 in vitro. This inhibition effects were redox-dependent and did not involve stable physical interaction. The data suggested a novel mechanism for redox regulation of MAPKs in plants.